Title: Evaluation of the Role of Dehalococcoides Organisms in the Natural Attenuation of Chlorinated Ethylenes in Ground Water 
Resource Type: document --> technical publication --> report 
Country: USA 
Year: 2007 
Availability: EPA 600-R-06-029 
Author 1/Producer: US EPA 
Author / Producer Type: Agency, regulator or other governmental or inter-governmental body 
Report / download web link (=direct link): http://www.epa.gov/ada/download/reports/600R06029/600R06029. ...  
Format (e.g. PDF): PDF 
Size: (e.g. 20mb) 2.22 
EUGRIS Keyword(s): Contaminated land-->Remediation options-->MNA
Contaminated land-->Soil and groundwater processes-->Microbiology
Groundwater protection-->Groundwater processes-->Microbiology
 
Short description: At most hazardous waste sites where monitored natural attenuation (MNA) of chlorinated solvents in ground water is successful as a remedy, the chlorinated solvents are biologically degraded to harmless end products such as ethylene or ethane. Many organisms can degrade chlorinated solvents such as tetrachloroethylene or trichloroethylene, to dichloroethylene and vinyl chloride. This contributes little to risk reduction because vinyl chloride is more toxic and more carcinogenic than tetrachloroethylene or trichloroethylene. The only organisms known to degrade dichloroethylenes and vinyl chloride to ethylene or ethane are members of the Dehalococcoides group. As a result, these organisms have a critical role in the evaluation of MNA at chlorinated solvent sites. In recent years, biochemical assays for the presence of DNA from the organisms have become commercially available. These assays are based on the polymerase chain reaction (PCR) for the amplification of DNA extracted from ground water. They are very sensitive and can be very specific. 
Link to Organisation(s): EPA Environmental Protection Agency
 
Submitted By: Professor Paul Bardos WhoDoesWhat?      Last update: 16/04/2007

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