Monitoring of polychlorinated biphenyls (PCBs) in ground waters through an immunochemical biosensor with electrochemical detection
Country: EU Projects
Start Date:   1/5/1994         Duration: 36 months         Project Type: RTD
Contract Number: EV5V-CT940407
Organisation Type:  University research group / research institute
Topics: 
Contaminated land-->Site investigation-->Methods
Contaminated land-->Soil and groundwater processes-->Geochemistry
Diffuse pollution-->Diffuse pollution overview
Diffuse pollution-->Monitoring
Groundwater protection-->Groundwater protection overview
Groundwater protection-->Monitoring-->Monitoring overview
Project objectives: 
To develop immunochemical biosensors with electrochemical detection based on enzyme-antibodies and enzyme-PCB conjugates with glucose oxidase (GOD), 
alkaline phosphatase (AP) and/orperoxidase (P) enzymes. Further objectives included the devlopment of disposable strips for enzyme activity measurement, 
development of a flow injection system for continuous monitoring, the development of a portable instrument emloying the above techniques and to compare the 
output of each system.
Project Summary: 
The programme had eleven different tasks:    
1: Production of electrochemical biosensor for enzyme activity determination    
2: Prodction of immobilised antigens or antibodies    
3: Development of electrochemical biosensors for PCB with immobilised antigens    
4: Validation of PCB biosensors in comparison in order to establish reference procedures    
5: Development of a continuous monitoring procedure    
6: Construction of a prototype for continous monitoring of PCBs in the field    
7; Validation of the continuous monitoring with biosensors using established reference procedures    
8: Development of disposable strips with immobiolised PCBs    
9: Development of a procedure for measuring PCBs with disposable strips    
10: Development of a prototype for PCB analysis with disposable strips    
11: Validation of disposable strip measurements using established reference procedures
Achieved Objectives:
Product Descriptions:
Additional Information: 
Lead Institution: 
Universita degli Studi di Firenze, Firenze, Italy  
Partner INstitutions: 
ENEL - Societa per Azioni, Brindisi, Italy  
Universidad Autonoma de Barcelona, Barcelona, Spain  
Frauenhofer-Gesellschaft zur Forderung der angewaden Forschung e.V. Munster, Germany  
 
This information was taken from a source other than the project site: European Commission (1994) Environment Research Programme, Catalogue of Contracts 
1992-1994: Technologies and Engineering for the Environment. ISBN 92-826-8280-3
Project Resources:
Weblink:
Funding Programme(s): 
EC Framework 4 Funding Programme FP4 of European Community activities in the field of research and technological development and demonstration
Link to Organisations:
Submitted by: EUGRIS Team Dr Antony Chapman  Who does what?  18/11/2005 14:27:00
Updated by: EUGRIS Team Professor Paul Bardos  Who does what?  28/09/2006 17:25:00