| Title: |
Determination of estrogens and progestogens by mass spectrometric techniques (GC/MS, LC/MS and LC/MS/MS)
|
| Resource Type: |
document --> technical publication --> journal article
|
| Country: |
EU Projects
|
| Year: |
2003 |
| Availability: |
Díaz-Cruz, Silvia et al. (2003) Determination of estrogens and progestogens by mass spectrometric techniques (GC/MS, LC/MS and LC/MS/MS) J. Mass Spectrom. 2003; 38: 917–923
|
| Author 1/Producer: |
Díaz-Cruz, Silvia
|
| Other Authors/Producers: |
López de Alda, Maria J.
López, R.
Barceló, D.
|
| Author / Producer Type: |
EC Project
|
| Format (e.g. PDF): |
PDF
|
|
EUGRIS Keyword(s): |
Contaminated land-->Contaminants-->Contaminants overview
|
| Short description: |
Steroid sex hormones and related synthetic compounds have been shown to provoke alarming estrogenic
effects in aquatic organisms, such as feminization, at very low concentrations (ng/L or pg/L). In this
work, different chromatographic techniques, namely, gas chromatography/mass spectrometry (GC/MS),
liquid chromatography/mass spectrometry (LC/MS) and liquid chromatography/tandemmass spectrometry
(LC/MS/MS), are discussed for the analysis of estrogens, both free and conjugated, and progestogens, and
the sensitivities achieved with the various techniques are inter-compared. GC/MS analyses are usually
carried out after derivatization of the analytes with bis(trimethylsilyl)trifluoroacetamide (BSTFA). For
LC/MS and LC/MS/MS analyses, different instruments, ionization techniques (electrospray (ESI) and
atmospheric pressure chemical ionization (APCI)), ionization modes (negative ion (NI) and positive ion
(PI)) and monitoring modes (selected ion monitoring (SIM) and selected reaction monitoring (SRM)) are
generally employed. Based on sensitivity and selectivity, LC/ESI-MS/MS is generally themethod of choice
for determination of estrogens in the NI mode and of progestogens in the PI mode (instrumental detection
limits (IDLs) 0.1–10 ng/mL). IDLs achieved by LC/ESI-MS in the SIM mode and by LC/ESI-MS/MS in the
SRM mode were, in general, comparable, although the selectivity of the latter is significantly higher and
essential to avoid false positive determinations in the analysis of real samples. Conclusions and future
perspectives are outlined.
|
|
Submitted By:
|
Dr Stefan Gödeke WhoDoesWhat?
Last update: 14/02/2006
|
|
|
