| Title: |
Integrated procedure for determination of endocrine-disrupting activity in surface waters and sediments by use of the biological technique recombinant yeast assay and chemical analysis by LC–ESI-MS
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| Resource Type: |
document --> technical publication --> journal article
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| Country: |
EU Projects
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| Year: |
2004 |
| Availability: |
Céspedes, R. et al. (2004) Integrated procedure for determination of endocrine-disrupting activity
in surface waters and sediments by use of the biological technique
recombinant yeast assay and chemical analysis by LC–ESI-MS. Anal Bioanal Chem (2004) 378 : 697–708
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| Author 1/Producer: |
Céspedes, R.
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| Other Authors/Producers: |
Petrovic, M., ·Raldúa, Demetrio ·
Saura, Úrsula, · Piña, Benjamín · Lacorte, Sílvia ·
Viana, Paula, · Barceló, Damià
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| Author / Producer Type: |
EC Project
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| Format (e.g. PDF): |
PDF
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EUGRIS Keyword(s): |
Contaminated land-->Contaminants-->Contaminants overview
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| Short description: |
An integrated procedure using mass spectrometry
and molecular biology for determination of estrogenicity
in natural waters and sediments is reported. Solid-phase
extraction (SPE) and pressurized-liquid extraction (PLE),
respectively, were used for isolation of endocrine-disrupting
compounds (EDC) from surface waters and sediments,
followed by liquid chromatography–mass spectrometry
using an electrospray interface (LC–ESI-MS). Twenty
seven EDC were determined: non-ionic surfactants (nonylphenol
ethoxylate), alkylphenols (e.g. nonylphenol and
octylphenol), bisphenol A, phthalates, and natural and synthetic
steroid sex hormones. Limits of detection varied from
0.02 to 0.22 µgL–1 and from 1 to 10 µgkg–1 in water and
sediments, respectively. Recoveries ranged from 65 to 125%
and 73 to 97% for waters and sediments, respectively. In
addition to LC–ESI-MS determination, extracts obtained by
SPE and PLE were analyzed by the recombinant yeast assay
(RYA) to assess total estrogenic activity. This bioassay
detects natural estrogens and xenoestrogens, producing a
quantitative measurement of EDC irrespective of the identity
of the chemical responsible for the activity. As a novelty,
a relative estrogenicity factor was determined for 19 analytes
with EC50 values ranging from 10–10 to 10–9 mol L–1
for synthetic estrogens, from 10–7 to 10–5 mol L–1 for
alkylphenol derivatives, and from 10–5 to 10–4 mol L–1 for
phthalates and benzothiazoles. By use of this integrated
chemical–ecotoxicological approach good correlation was
usually established between chemical composition and estrogenic
effects for surface water and sediment samples
from Portugal. Estrogenic activity observed was mainly attributed
to the presence of nonylphenolic compounds (with
concentrations of NP ranging from 0.1 up to 44 µgL–1 in
waters and up to 1172 µgkg–1 in sediments), and to the
sporadic presence of estrogens, detected at ng L–1 levels.
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Submitted By:
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Dr Stefan Gödeke WhoDoesWhat?
Last update: 14/02/2006
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